Sentence examples for by second generation sequencing from inspiring English sources

The BAC map is used for sequencing single BACs or BAC-pools covering defined genomic entities by second generation sequencing technologies.

All genes in this 5 Mb genomic region were enriched using a custom sequence capture array from NimbleGen followed by second generation sequencing (a Genome Analyzer II from Illumina).

Extrapolating from these observations, we suggest that, in cases of FADS/LMPS, RYR1 mutation should be performed as part of a multigene diagnostic strategy (e.g. by second generation sequencing analysis) rather than being specifically targeted to cases with histopathological features that are considered characteristic of a RYR1-associated myopathy.

Data obtained at this stage will be used to establish a new methodological workflow for the 5mC and 5hmC analysis at DREs using capture array for fragment enrichment followed by third generation sequencing.

Other programs, such as Velvet assembler, have been optimized for the shorter reads produced by second-generation sequencing through the use of de Bruijn graphs.

These candidate genes can be sequenced by second-generation sequencing technologies, leading to the detection of many potentially causative SNP that probably include the causative mutation.

Alternatively, laser-capture microdissection followed by second-generation sequencing may be used for rare transcript identification, but the process is technically challenging [ 28, 29].

At the time of writing, the length of the reads obtained by second-generation sequencing (e.g. Illumina Genome Analyzer) are limited to about 100 bases and the sequencing occurs in the 5′→3′ direction.

Summary: We present bammds, a practical tool that allows visualization of samples sequenced by second-generation sequencing when compared with a reference panel of individuals (usually genotypes) using a multidimensional scaling algorithm.

With the tremendously increasing number of prokaryotic genomes that is being sequenced, there is a clear need for automated prokaryotic genome annotation tools able to integrate the variety of informative data that can be produced either by second-generation sequencing or by other high-throughput analyses, such as tiling arrays and proteomics.

Next-generation sequencing (NGS) is related to the Sanger sequencing method, which is represented by first-generation sequencing technologies.