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By screening for modifiers of many different perturbations, we are able to quantitatively partition the effects of these mechanisms.
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Liu et al. discovered the transcription factor Spt4/Supt4h when screening for modifiers of the toxicity of proteins carrying expanded polyQ stretches.
By screening for alterations of a dominant wing vein phenotype we have found modifiers of the DGC that are involved in cytoskeletal organization.
The hyperplastic eye phenotype produced by driving UAS-Ras V12 with ey-GAL4 was screened for modifiers using a collection of P-element enhancer insertion lines bearing UAS promoter sequences.
Several models of HD are being used to screen for modifiers of mutant polyQ toxicity and for genes dysregulated by mutant htt.
A collection of approximately 3650 P element insertion lines was screened for modifiers of stbm.
A collection of 216 deficiency lines were screened for modifiers of act-Gal4:UAS-DysN-RNAi/CyO mutant.
This model can now be used to screen for modifiers of FAS that might help to prevent or treat the syndrome in humans.
In addition, exhaustive phenotyping was performed in all patients carrying mutation(s), and the AHI1 gene was screened for modifier alleles of CEP290-related disease.
Screening for genetic modifiers as well as testing of combinatorial therapies could be performed using the Drosophila model established here.
Our GeneMapper quantification method is readily applicable to high-throughput assays such as screening small molecules that modulate instability in cells, or screening for genetic modifiers in mice.
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