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Gaps were created artificially by scratching the cell monolayer.
A wound was created by scratching the cell monolayer with a sterile 200 μL pipette tip.
A gap of ∼1 mm wide was generated by scratching the cell monolayer with a sterile tip.
Following confluence a thin wound (800 μm) was introduced by scratching the cell monolayer with a sterile pipette tip.
A thin wound (800 μm) was introduced by scratching the cell monolayer with a sterile pipette tip.
A line of cells was mechanically removed by scratching the cell layer with a 200 μl pipette tip to create a 'wound'wound
Similar(53)
Primary hepatocytes were plated on collagen I-coated 6-well plates (3×105 cells/well) and then "wounded" by scratching the cells with a 200 µl pipette tip in the presence or absence of TGF-β1 after attachment and then monitored in 24 and 48 h by phase-contrast microscope photography as described previously [23].
By mechanically scratching the cell substrate with a pin array, we are able to create characteristically sized wounds in all wells of a 384 well plate.
Cells were seeded in 6-well plate and grew to confluence followed by scratching the monolayer cells with a 200 μL pipette tip to create wound.
The linear wound of cellular monolayer was created by scratching the sub-confluent cell monolayer using a plastic pipette tip (200 μl).
Mouse bone marrow cells and the cells collected by scratching the inner layer of the esophagus and stomach of both untreated and treated for 260 days with RAN with or without lime were fixed with 70% ethanol.
More suggestions(17)
by scratching the cells
by scratching the dish
by scratching the pin
by scratching the monolayer
by scratching the bottom
by scratching the material
by scratching the end
by scratching the coating
by scratching the plate
by scratching the overcoat
by scratching the sample
by scratching the nanoparticle
by scratching the screen
by scratching the telecast
by scratching the carpet
by scratching the organ
by depriving the cell
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