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X-ray films were quantified by scanning densitometry using ImageJ software (National Institutes of Health).
The intensity of the bands was quantified by scanning densitometry using software Quantity One-4.5.0.
Relative intensities of the protein bands were quantified by scanning densitometry using ImageJ.
Protein bands on these images were analyzed by scanning densitometry, using Molecular Analyst program (BioRad, Hercules, CA, USA).
Specific bands were quantified by scanning densitometry using the Image J1.34 software (NIH, Bethesda, MD, USA) version 1.61.
The bound antibodies were visualized by chemiluminescence detection and protein levels were quantified by scanning densitometry using TotalLab© software.
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Data were acquired by scanning-densitometry using a Chemi-Start (Vilber-Lourmat, Marne-La-Vallée, France).
The protein signals were quantified by scanning densitometry by using a bio-image analysis system (Bio-Profil Celbio, Milan, Italy) and were expressed as integrated intensity in comparison with those of control normal animals measured with the same batch [ 17].
The relative level of expression for each gene has been calculated as the ratio between the signal obtained in the area of the target band and the signal of the GAPDH, by scanning densitometry by using the GELDOC XRS system by the QuantiOne 1-D analysis software (BIORAD, Richmond, CA, USA).
The films were quantified by computer-assisted scanning densitometry using UN-SCAN-IT software (Silk Scientific, Orem, UT).
Band intensities of HIF-1 α were quantified by scanning densitometry and analysed using ImageJ software.
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