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Such convenience-within-probability surveillance could provide statistically valid estimates of disease absence and prevalence by reducing the effect of bias generated by sampling on a first-come-first-served basis.
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A total of 50 participants were interviewed by convenience sampling on a one-to-one, non-directive basis (Maree 2009).
Generate a sample by sampling from a proposal distribution based on the current state.
A lineage experienced the survival assay at 45°C, followed by sampling to create a dilution series on host lawns.
Because very few soil point observations were available within the area, we calibrated the regression model by sampling the soil map on a grid.
RNA concentration was estimated by spectrophotometry and integrity was confirmed by running samples on a 1.2% denaturing agarose gel.
RNA concentration was estimated by spectrophotometry (GeneQuant, GE Healthcare); integrity was confirmed by running samples on a 1.2% agarose gel.
The integrity of RNA was determined by running samples on a 1.3% agarose gel.
The quantity of RNA was determined using the Qubit assay (Qubit® 2.0 Fluorometer, Life Technologies and RNAA integrity was assessed by running samples on a 1.5 % agarose gel.
The degree of fragmentation and the length distribution of this fragmented biotinylated aRNA was checked by running samples on a 1.0% agarose gel.
RNA-seq libraries were constructed using the TruSeq™ RNA sample preparation kit (Illumina, San Diego, CA) and their quality determined by running samples on a high-sensitivity DNA chip on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
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