Sentence examples for by sampling fluorescence at from inspiring English sources
By sampling fluorescence at low laser power at the sample, we were able to collect up to about 2×105 scan lines (corresponding to approximately 90 s) with no detectable photobleaching on the sample.
The flow of Ca2+ ions can then be inferred by sampling the fluorescence levels at the site of each nanopore.
Cell viability was quantified by measurement of the sample fluorescence intensity at 560EX/590EM.
A differential profile was then evaluated for each sample by comparing fluorescence at the melting point with the fluorescence of the unmethylated control.
At 0 and 72 h of growth, samples were stained with Nile red and the lipid content of the culture was evaluated by measuring the sample fluorescence by fluorescence microscopy and flow cytometry.
Indirect measures of NCqs are estimated by fluorescence sampling.
The temperature was maintained at 55°C for 15 sec to analyze sample fluorescence, and the temperature was increased by increments of 0.5°C followed by 15 sec of fluorescence analysis for a total of 80 cycles.
Fluorescence ratio is, PKH67 fluorescence at D0 divided by PKH67 fluorescence at D4 (D4/D4).
Samples were analyzed by fluorescence spectrophotometry at the excitation/absorption wavelengths recommended by manufacturer for GFP detection and quantification (Cary 300, Varian).
After washing samples were analysed by fluorescence microscopy at ×20 magnification (Solent Scientific, Segensworth, UK).
