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Statistical analysis of mean percentage of strong staining by sample group was conducted using ANOVA followed by the Newman Keuls multiple comparison test.
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The sample group was obtained by purposive random.
The sample group was composed by 3,173 subjects from two Italian Regions, Campania and Lazio.
The number of osteoclasts after 48 hours in each sample group was counted by analyzing multinuclear TRACP-positive cells in the resorption assay.
Then, the number of CD20+ cells in each sample group was determined by co-staining with fluorescein isothiocyanate (FITC -conjugated anti-CD20 (BioLegend).
Participant characteristics by site, sex and sample group are presented in Table 1.
Protein expression in the sample groups was demonstrated by western blot analyses.
The division of the two sample groups was supported by a 100% bootstrap value after 1,000 permutations.
Each sampling group was represented by 4 castrates and 4 females.
Data involving the analysis of multiple sample groups were analyzed by one-way ANOVA followed by Bonferroni's test for multiple comparisons.
Mean and median methylation levels for sample groups were compared by one-way ANOVA, followed by Tukey's test for the significance of differences.
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