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Spectra can be conveniently split into those obtained during 2002 (listed by sample and date in Table 1, Additional File 1) and the Helios screening run (listed in Table 2, Additional File 1).
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Samples were collected on nine different occasions and were designated by sample identifier and date as follows: A-February 2002, B-March 2002, C-June 2002, D-July 2002, E-September 2002, F-October 2002, G-March 2003, H-April 2003, and I-June I-June 2003
Mean concentrations of E2, 11-KT, and VTG in males and females, categorized by sampling date and stage of maturation, are summarized in Tables 2 and 3. When only one or two fish were at a particular stage of maturation, the data were not included in the tables.
The relevant samples from each dataset were further organized by sampling date and sampling locations, then grouped accordingly into spacecraft "zones".
Both DEA (df = 2, 134; F = 14.15; p<0.0001; Figure 1A) and mass-standardized DEA (df = 2, 134; F = 10.11; p<0.0001; Figure 1B) varied significantly by sample date, with significantly higher values in mid-July than early May and late August.
The distribution of MRSA-positive samples differed by WWTP, sampling date, and sampling location (Table 1).
The distribution of MSSA-positive samples differed by WWTP, sampling date, and sampling location (Table 2).
This correlation is confounded by sampling date as we inadvertently sampled the two high nitrate springs (Fanning and Ruth) first.
In contrast, copies of nosZ increased with urine addition (P < 0.05) but were unaffected by sampling date (increasing soil temperature).
The results indicated that the most likely number of cryptic subpopulations was four, and these differed significantly by FST and Nei's genetic distance, previously masked due to the subpopulations being mixed when arbitrarily partitioned into populations by sampling date or host species.
The number of samples collected varied by sampling date, ranging from 57 to 100.
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