Sentence examples for by running a gradient from inspiring English sources

The values of the coefficients are determined by running a gradient descent method.

DFE-WF, Θ∗, for every sample of the channel is obtained by running a gradient descent type of algorithm on the cost function (4) itself, where the gradient was approximated at each point by finite difference approximation, [EΘ + Δ(X t −s]2−E Θ (X t −s)2) /|Δ|.

Dissociation analysis of the PCR products was performed by running a gradient from 60 to 95°C to confirm the presence of a single PCR product.

The purification was achieved by running a gradient from 0 to 50% 1M NaCl in 30mM TRIS, pH 8.0 in 16 CV at 5 ml/min.

Bound proteins were then eluted from the column by running a gradient from 0 to 50% of 500 mM Imidazole, 0.3 M NaCl, 50 mM Na phosphate buffer, pH 8.0 in 12 CV.

Separation was obtained by running a gradient at a 2-μL/min flow rate.

Condition the column by running a RP gradient through it and ending with Buffer A wash.  .

Condition the column by running a RP gradient through it and ending with Buffer A wash. i.

The extract was applied on Q Sepharose FF pre-equilibrated with buffer A (50 mM Tris-HCl, pH 7.4), and bound materials were eluted by running a NaCl-gradient (0→1 M) in buffer A. Various nuclease activities were detected after separation, and the double-strand (ds) specific activity addressed in this report eluted at 0.4 M NaCl.

In the second stage of the fitting process, a depth profile and corresponding error bars are extracted from the experimental spectrum by running a generalized reduced gradient (GRG2) algorithm using the previously calculated multilayer target.

The separation was performed by running a non-linear gradient: 4% B, in 0.1~3.5 min for injection; 4~50% B, in 3.5~63.5 min; 50~100% B, in 63.5~73.5 min. The CapLC is coupled on-line with a Q-TOF Micro maspectrometerter (Micromass, Manchester, UK) for detection and protein identification.