Soon, we shall see the advent of telesurgery for routine procedures using robots and nurse assistants.
The specimens were processed according to routine procedures, using Delaunay's solution as a fixative.
Flow cytometry followed routine procedures using 1−3 × 10 tumour cells per sample.
Clinical specimens were collected per routine procedures using the BD SurePathTM liquid-based Pap test.
Peripheral blood cells can be isolated from patients by the same routine procedure used for obtaining whole blood, serum, and plasma and thus serve as prime candidates for clinical studies of samples directly obtained from patients.
No more information than those collected by routine procedures was used; in particular, no additional individual data, biological collection or sample was required.
Archival normal and neoplastic mammary tissue that had been fixed in buffered 10% formalin for 24 to 72 h, sent to the Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich by the Unit of Small Animal Reproduction, Vetsuisse Faculty, University of Zurich, and embedded in paraffin by routine procedures was used.
Here, we introduce a novel automated imaging analysis routine, based on Gaussian, followed by a segmentation procedure using CellProfiler software (www.cellprofiler.org).org
routines and followed the procedures used in our previous publications (Baker and Baker, 2003; Riddle et al., 2004).
The coefficients of the bilinear curves were computed by a least-square nonlinear fitting routine using the Levenberg-Marquardt procedure (OriginLab, version 9.1.0).
