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We therefore analyzed pannus tissue from RA patients and compared the obtained protein pattern with that from tissues derived from patients with osteoarthritis (OA) first by RNA microarray-analysis (Affymetrix) [ 3, 4].
Immunoprecipitation of RNA binding proteins and identification of the associated cellular RNAs by microarray analysis (often called "RIP-CHIP") has been successfully used by a number of groups (Reviewed in [25]).
Altered transcription of components of the Akt/GSK3-β regulatory pathway was consistently observed in flies expressing rCAG, rCUG and rAUUCU repeat RNAs by microarray analysis, suggesting that this is a key component of cellular dysfunction in this Drosophila model of untranslated repeat disease pathogenesis.
Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis.
A putative small RNA identified by microarray analysis was amplified by qPCR using an miScript PCR System (Qiagen) with a set of primers (M1 to M7 in Additional file 2: Table S4) that spanned the potential miRNA sequence.
The Brucella RNA for microarray analysis was purified and amplified by the MessageAmp II-Bacteria RNA Amplification Kit (Ambion), which enables prokaryotic RNA amplification for whole genome expression analysis from bacterial samples.
This assumption has been challenged by Trask et al.[ 3], who demonstrated that the nuclear contribution does impact the gene expression profile when examining steady-state messenger RNA (mRNA) by microarray analysis.
RNA for microarray analysis was isolated at this time point.
RNP participated in RNA isolation, microarray analysis and ontology analysis.
Differential expression of selected genes observed by RNA-seq or microarray analysis was confirmed using RT-qPCR or Northern hybridizations.
Cyp1a1 exhibited a 771- and 82-fold induction, while Sult3a1 was repressed 100- and 25- fold by RNA-Seq and microarray analysis, respectively.
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