Sentence examples for by reversing sequences from from inspiring English sources
A second decoy (random) database was constructed by reversing sequences from the normal database.
Spectra were searched using Mascot (version 2.3) against three appended databases consisting of: i) C. elegans protein sequences (WS220); ii) common lab contaminants; and iii) a decoy database generated by reversing the sequences from these two databases.
The data sets were searched against both the human Swissprot 2012_09 database, and a decoy database obtained by reversing the sequences from the human database.
A consensus sequence was computed from the forward and reverse sequences with SeqMan version 8 from the Lasergene package (DNASTAR).
All templates were sequenced for 76 cycles from both ends on the Illumina GAII platform, and reads merged into single fragments were included in subsequent analyses only if forward and reverse sequences overlapped by a minimum of 11 bp.
Forward and reverse sequences were assembled by using Sequencher (GeneCodes).
Sequences of putative lncRNAs and mRNAs were excised from the genome with samtools, and reverse sequences were reversed and complemented with the revseq –reverse –complement command.
All forward sequencing results were confirmed by reverse sequencing.
Several Rad51 variants were identified and confirmed by reverse sequencing.
