Sentence examples for by restriction fragment analysis from inspiring English sources

HPV genotypes were determined by restriction fragment analysis of PGMY09/PGMY11 polymerase chain reaction (PCR) products using seven restriction endonucleases.

The resulting fragments and templates were successfully ligated, generating pAGS7, which was validated by restriction fragment analysis using NsbI.

These amounts were insufficient to perform further comparative analyses by restriction fragment analysis or Southern blotting.

Mapping of BAC clones was performed by restriction fragment analysis with NotI and NruI (NEB).

Vectors were verified by restriction fragment analysis and/or DNA sequencing.

Genome types were assigned by restriction fragment analysis with 12 enzymes as in the previous study (9).

The linear regression line between mean telomere length (measured by terminal restriction fragment analysis based on Southern blotting) and the T/S ratio (measured by the quantitative PCR based method) was obtained, as described previously (14, 16), and used for the calculation of the corresponding telomere length in base pairs (bp) from the T/S ratio measured in each subject.

The resulting fusion gene of SV-F and GP64, Sendai-GP64, was verified by both restriction fragment analysis and sequencing.

The presence of an insert of the correct size was confirmed by EcoRI restriction fragment analysis in the vector pCR4-TOPO.

Our observations thus match data of mean telomere lengths of over 50 kb, as measured by terminal restriction fragment analysis in so-called human ALT-positive cell lines [ 18].

Each of the 10 plates also included three samples isolated from a series of cell lines with known telomere lengths, ranging from 3 to 15 Kb, as determined by terminal restriction fragment analysis.