Sentence examples for by restriction analysis using from inspiring English sources
The presence of the new insert was confirmed by restriction analysis using SmaI.
bValidated by restriction analysis using HphI.
cValidated by restriction analysis using DpnI and DpnII.
Plasmid DNA from clones was isolated using High Pure Plasmid Isolation Kit Roche Applied Sciencee) and tested by restriction analysis using HindIII enzyme.
Plasmid DNA from white clones was isolated using High Pure Plasmid Isolation Kit (Roche Applied Science) and tested by restriction analysis using FastDigest® NotI enzyme (Fermentas, Hanover, Maryland, USA) to identify whether they contained GSL gene inserts.
Isolated cDNA products (4 μL) were cloned into pCR2.1-TOPO vector using the TOPO TA Cloning Kit (Invitrogen Canada Inc .. Plasmid DNA was purified from selected transformants using the QIAprep Spin Miniprep Kit (Qiagen, Mississauga, Ontario, Canada), and the presence of insert sequence was confirmed by restriction analysis using Eco RI (Amersham Biosciences).
To confirm if the collected cells were predominantly germ cells, we checked the DNA methylation level of the PEG10 DMR by COBRA (combined bisulphite restriction analysis) using genomic DNA extracted from the collected cells.
After antibiotic selection (G418/Geneticin; Gibco-BRL, Grand Island, NY, USA), homologous recombinants were identified by PCR using the set of primers named 'screen2 F' and 'NeoLOXP2 R' shown in Table 2. Homologous recombination was confirmed by Southern blotting and extensive restriction analysis, using flanking and internal probes (data not shown).
The PCR products were subjected to restriction analysis using HinfI restriction endonuclease (New England Biolabs, Massachusetts, USA) at 37 °C followed by electrophoresis in a 2.5% agarose gel.
The resulting fragments and templates were successfully ligated, generating pAGS7, which was validated by restriction fragment analysis using NsbI.
The presence of the D816V mutation was also confirmed by restriction digestion analysis using BsmA1 and Ple1, which detect the wild-type and mutated form of KIT, respectively.
