Sentence examples for by restriction analysis based from inspiring English sources

The gB genotype of CMV was determined using the polymerase chain reaction to amplify a region of UL55, followed by restriction analysis based on HinfI and RsaI digestion.

Positive samples from patients with active CMV infection were submitted to genotyping using N-PCR to amplify a region of UL55, followed by restriction analysis based on HinfI and RsaI digestion.

The linear regression line between mean telomere length (measured by terminal restriction fragment analysis based on Southern blotting) and the T/S ratio (measured by the quantitative PCR based method) was obtained, as described previously (14, 16), and used for the calculation of the corresponding telomere length in base pairs (bp) from the T/S ratio measured in each subject.

The type of chromosomal rearrangements present in the various selected revertants was determined by performing a BamHI restriction pattern analysis based on Southern blot hybridization.

By restriction analysis we showed that pVMB5 contains a 2.2 kb TaqI insert where a 600 base pairs (bp) streptococcal DNA fragment was cloned upstream of the cat gene.

Correct methylation was checked by restriction analysis with Fnu 4HI.

Orientation and codon in-frame for all constructs were confirmed by restriction analysis and sequencing.

16S rRNA and DNA PCR products were cloned and screened by restriction analysis.

Constructs were confirmed by restriction analysis and DNA sequencing.

The resulting clones were confirmed by restriction analysis and sequencing.

All Entry clones were verified by restriction analysis and sequencing.