We further investigated the distribution of these two specific cidA/cidB variants [cidA_IV and cidB_IV(a/2)] at a larger scale, by performing diagnostic restricted fragment length polymorphism (RFLP) tests on 180 isofemale lines from 15 populations [13 in Algeria and Tunisia; 1 each in China and Turkey], for which we determined crossing types.
We combined demographic, clinical and epidemiologic information with IS6110-based restricted fragment length polymorphism (RFLP) and Spoligotyping analysis of MTB isolates.
A total of 184 RILs were genotyped for 488 molecular markers (restricted fragment length polymorphisms, simple sequence repeats and amplified fragment length polymorphisms) that construct a linkage map with 25 linkage groups covering 4,151.2 cM of the soybean genome [56].
Only studies that used validated genotyping methods, such as direct gene sequencing, polymerase chain reaction paired with restricted fragment length polymorphism and denaturing gradient gel electrophoresis, were included.
Another PCR, a restricted fragment length polymorphism (PCR-RFLP) was performed on all the specimens diagnosed as An.
The clones with the desired recombination were selected on kanamycin- and L-arabinose-containing plates and then subjected to PCR and restricted fragment length polymorphisms (RFLP) analysis.
Phylogenetic grouping was performed using a rapid method combining gyrA PCR- restricted fragment length polymorphisms analysis (RFLP), parC-PCR and adonitol fermentation as described previously [ 16].
A major cause of these disease problems was attributed to the emergence of a different strain of porcine circovirus type II (PCV-2), restricted fragment length polymorphism (RFLP) pattern 321 [ 1].
38 Moreover, this SNP assay was validated using an alternative restricted fragment length polymorphism-based genotyping method (n = 476 samples) with 100% concordance, suggesting significant genotyping error is unlikely.
The map was constructed using restricted fragment length polymorphism (RFLP), simple sequence repeats derived from expressed sequence tags (EST-SSR), single nucleotide polymorphism/insertion and deletion (SNP/INDEL), intron fragment length polymorphism (IFLP), targeted region amplification polymorphism (TRAP) and inter-simple sequence repeat amplification (ISSR).
