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We also sought to confirm the identity of rhTyrRS by resolving the sample (s) in parallel with a reference standard by SDS-PAGE followed by western blotting using a polyclonal antibody.
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Endogenous LC3 lipidation and 4E-BP1 phosphorylation were assessed by resolving protein samples on a 13.5% acrylamide gel with 0.36% methylene bisacrylamide.
The PCR product sizes were verified by resolving samples on agarose gels and PCR products were sequenced to confirm the identity of each target.
RNA integrity was estimated by resolving 1 μg of sample in a 1%% (m/v) ethidium bromide agarose gel.
In order to check film composition and element distribution along the film thickness, XPS measurements were performed in a depth resolved configuration by etching the sample surface with Ar+ ions and an example is shown in Fig. 1(a), where the presence of Ar peaks is related to the etching process.
The products were not well resolved by LC MS, so the sample was reanalyzed using IMS MS.
This issue of variation may be resolved in future studies by increasing the sample size.
The control system is developed to resolve the issues caused by a short sampling time, which is required to meet the refractory control objectives of the modern RTP system (e.g., rapid ramping and a high degree of temperature uniformity).
Each of these chirps is cross-correlated with the PT image (S) captured by the camera in a pixel-by-pixel manner to produce two-dimensional axially resolved layer-by-layer images of the sample.
After resolving samples by 10% SDS PAGE, the gel was stained with Coomassie blue to check for equal loading, then dried and exposed.
Both with and without a reduction by β-mercaptoethanol, the samples were resolved by SDS-PAGE (Lonza, Rockland, ME) and gels were negatively stained with copper chloride.
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