Jones et al. tried to optimize a protocol for deriving reproducible and representative gene expression profiles by microarrays from very rare samples of human oocytes, available for research purposes [ 22].
The Roche Nimblegen Whole Human Genome Expression profile microarray was used to detected representative gene expression profiles of hDPSCs by ginsenoside Rg1.
The module eigengene is a representative gene expression profile that summarises the expression profiles of all genes in the module.
The differential protein expression encoded by representative genes that suppress (LXN, TFF1, and GATA3) and stimulate (FABP4 and FABP6) carcinoma progression (Ohmachi et al, 2006; Kouros-Mehr et al, 2008; Li et al, 2011; Nieman et al, 2011; Soutto et al, 2011) was substantiated at the protein level.
To further validate the results from the SHH experiment and to quantify expression of genes involved in starch biosynthesis and/or carbon portioning, we chose 19 genes as representatives for gene expression analysis by qPCR, including two reference genes, eEF-1 α and UBQ5 (Additional file 5).
This was achieved by summarizing gene expression profiles representative of a given KEGG pathway with the first two principal components (PCs) of the gene expression matrix [14].
Next, the significance of motif representations was evaluated by analyzing gene expression correlations in representative cancer datasets.
For such modules, one can summarize the module expression profile by one representative gene: the module eigengene.
