Sentence examples for by replacing the native from inspiring English sources

We constructed a recombinant E. coli strain by replacing the native NAD-dependent gapA gene with a NADP-dependent GAPDH from Clostridium acetobutylicum, encoded by the gene gapC.

In this study, we draw on the experiences described in vector pFGL815 N and pCeGFP, and devised a novel strategy for site-specific integration of foreign DNA via carboxin resistance reconstitution by replacing the native Mosdi1 with Mosdi1 R allele.

The two novel diastereoisomeric glutathione analogues 1 and 2 have been designed and synthesized by replacing the native γ-glutamylic moiety with the conformational rigid skeleton of cis- or trans-4-carboxy-l-proline residue.

Here we have increased the production of β-carotene in Escherichia coli by replacing the native promoter of the chromosomal isoprenoid genes with the strong bacteriophage T5 promoter (PT5).

Experimental control of target gene expression is desired and can be achieved by replacing the native promoter of the target gene with a tightly regulated promoter whose activity can be controlled by the researcher (Gomez and Bishai 2000; Chalut et al. 2006; Gandotra et al. 2007).

In order to increase the pool of malonyl-CoA, a key precursor in resveratrol biosynthesis, the acetyl-CoA carboxylase (ACC1) gene was additionally overexpressed in the yeast by replacing the native promoter of the ACC1 gene with the stronger GAL1 promoter and this resulted in enhanced production of resveratrol (4.3 mg/L).

By replacing the two alleles of the native promoter with the stronger TEF1p in situ, the Xks1p gene XKS1 was overexpressed (Additional file 1: Fig. S1c) (Peng et al. 2011).

The VCN construct was designed by replacing the C-terminal tail of native CN with the tail of another native disintegrin, echistatin, a short length viperid disintegrin.

We reengineered the native FliC structure by replacing the FliC D3 domain to contain a conformationally intact DENV2 E protein domain, without compromising either protein's structure.

To test further the importance of the Abf1 binding site for transcription initiation at select memory effect genes, Abf1 binding sites were mutated in promoters of three memory effect targets, RPL3, RPS28A, and CNB1, and one transcriptionally responsive gene, ARO3, in their native chromosomal context by replacing the recognition sequence with a sequence to which Abf1 cannot bind.

To increase free-energy conservation during fermentation of the industrially relevant disaccharide sucrose by Saccharomyces cerevisiae, we first replaced the native yeast α-glucosidases by an intracellular sucrose phosphorylase from Leuconostoc mesenteroides (LmSPase).