Sentence examples for by replacing the medium from inspiring English sources

The chemical may be introduced at a precise time, the dose can be altered over a large range and the chemical may be removed simply by replacing the medium surrounding the embryo.

By replacing the medium with fresh medium, cells were treated with CSSS, C-SLN and Tf-C-SLN (20 μM curcumin/well) for different time intervals (6, 12, 24, and 48 h).

500 μmol/L biotin-phenol (ApexBio) was added to cell medium for 30 min. Then 1 mmol/L of H2O2 was added and the plates were gently agitated for 1 min. The reaction was quickly quenched by replacing the medium with DPBS containing 5 mmol/L Trolox (abcam) and 10 mmol/L sodium ascorbate (Solarbio).

Unbound BrdU was removed by replacing the medium to DMEM.

Cells were released from the inhibitor by replacing the medium with warm, fresh one.

Then, proliferation of the cells was stimulated by replacing the medium with DMEM supplemented with 10% FBS.

The zinc level can be reduced by replacing the growth medium with medium without NPs (data not shown).

The cells were stimulated by replacing the culture medium with medium containing DEX.

Osteogenesis was induced by replacing the culture medium with osteogenic induction medium composed of culture medium supplemented with 10 mM β-glycerophosphate, 10 nM dexamethasone, 50 μg/ml sodium ascorbate 2-phosphate.

Cells were allowed to differentiate into myotubes by replacing the growth medium with differentiation medium (DMEM with 1% heat-inactivated horse serum, 100 µg/ml streptomycin sulphate, 100 units/ml penicillin-G) in 18% CO2 for 5 days.

After 3 days, the non-adherent cells were removed by replacing the spent medium with fresh medium.