After 3 days, the nonadherent cells were removed by replacing the medium with fresh complete medium.
This is done by replacing the null hypothesis of complete spatial randomness, with a null hypothesis that accounts for each individual's probability of being a case based on his/her known risk factors and covariates.
Chromatin complexes were cross-linked by replacing the culture media with fresh complete media containing 1% formaldehyde, and cells were incubated at room temperature for 10 min. Cross-linking was quenched by adding glycine to a final concentration of 0.125 M. Cells were washed two times with ice-cold PBS, and cell pellets were flash-frozen in a slurry of dry ice and ethanol.
With a recent result of Suzuki (2001) we extend Caristi-Kirk's fixed point theorem, Ekeland's variational principle, and Takahashi's minimization theorem in a complete metric space by replacing the distance with a -distance.
The optimal control law is obtained from minimizing the dynamical programming equation subject to the constraints of ER/MR damping forces, and the fully completed averaged Itô equations are obtained from the partially completed averaged Itô equations by replacing the control forces with the optimal control forces and by averaging the terms involving the control forces.
On becoming leader in 1994 he completed Labour's "modernisation" by replacing the bit of its constitution that called for common ownership of the economy with a commitment to the "enterprise of the market", the "rigour of competition" and a "thriving private sector"—as well as high-quality public services.
Acidic treatment was performed one hour later by replacing the culture medium with DMEM acidified with citric acid (Sigma-Aldrich) at pH 5.0 for 15 min. Medium was further aspirated and replaced by complete medium.
The leakage signal is either generated by one of two antenna configurations or by replacing this complete block with another CCU2 for artificial leakage generation.
This is achieved by simply replacing the first complete degenerate codon within any 5 base sequence repeat with an alternative synonymous codon regardless of any previously defined codon usage constraints.
