Sentence examples for by replacing the buffer from inspiring English sources

During incubation, the pH of the effluent was measured and maintained at 7.4 by replacing the buffer solution once every 4 days.

The samples were dehydrated by replacing the buffer with increasing concentrations of ethanol (from 40 to 100%) for 10 min each.

Simulated reperfusion was achieved by replacing the buffer with M199 medium containing 2 g/L BSA, 0.62 g/L taurine, 0.32 g/L carnitine, 10 units/mL penicillin, 10 mg/L streptomycin.

Reperfusion can then be simulated by replacing the ischaemic buffer with normal oxygenated buffer and removing the cells from the hypoxic chamber.

The infection was synchronized by replacing the Endo buffer by permissive medium (IMDM + 10 mM Hepes buffer, pH 7.4, and 5% FCS) for 2 min. Free parasites were subsequently removed by repeated washing with medium until no free parasites could be detected microscopically.

OGD was terminated by removing the culture from the hypoxia chamber, and by replacing the glucose-free buffer with the mixed media (1 1 mixture of fresh and conditioned media with 1  μM MK801).

Negative controls were processed in parallel by replacing the primary antibody by buffer.

A control reaction was prepared by replacing the ascorbate with reaction buffer.

Negative controls were carried out by replacing the primary antibody with buffer.

The reaction was stopped by replacing the substrate with sodium acetate buffer and the results were examined with a microscope.

Sections known to stain positively were included in each batch and negative controls were prepared by replacing the primary antibody with TBS buffer.