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Cells were incubated at 37°C in a humidified atmosphere of 5% CO2 for 6 h followed by replacement of complete medium.
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Remaining extracellular bacteria were then killed by replacement of the medium with complete medium containing 30 μg ml−1 gentamicin.
This dolomite was formed by complete replacement of carbonates during both early burial and late burial via hydrothermal basinal fluids.
This carcinomatous proliferation may occur as in situ or infiltrative growth, as demonstrated either by a loss in continuity of the myoepithelial and basal layers associated with the neoplastic cells invading the stroma or by complete replacement of the pre-existing benign lesion at the time of histopathological examination [ 9].
The H2be-GF transgenic mouse line, which expresses Flag-H2be under control of the olfactory marker protein (Omp) promoter, was generated by complete replacement of the Omp CDS in plasmid pJOMP (Danciger et al., 1989) with a sequence encoding FLAG-H2BE, followed by pronuclear injection (Harvard Genome Modification Facility) of the linearized construct into fertilized mouse zygotes.
To parameterize the model, we used three temporal variables, extracted from published data from field studies: period of persistence of a given cover type, the time interval of forest cover replacement by another, and the time of complete replacement.
In mammals, cardiac damage is not followed by the complete replacement of lost cellular components but is rather defined by a relatively minor capacity for regeneration and far more robust reparative response.
The RAC was formulated by the complete replacement of natural aggregates with recycled aggregates.
Although these developmental diseases are unlikely to be cured by cell replacement therapies, a complete picture of disease pathology by disease modeling will facilitate the discovery of effective compounds that can improve patient conditions [ 46].
The type 2 dolomites are characterized by the partial to complete replacement of muddy limestone/wackestone but rarely replace packstone that experienced early marine cementation (Fig. 5h), which resulted in a decrease in permeability.
In late mitosis, Cdc20 is degraded by Cdh1/APC and leads to the complete replacement of Cdc20/APC by Cdh1/APC [ 7, 8].
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