Sentence examples for by replacement of buffer from inspiring English sources

Also, trials were made by replacement of buffer by 0.1 M HCl or NaOH.

Butyrate was increased from 4 to 36 mmol/L in butyric buffer by replacement of acetate, and ammonia (NH3) was increased from 2.5 to 22.5 mmol/L in ammonia buffer by replacement of NaCl.

In the future it will also be possible to automate the replacement of buffer in the outer chamber, with obvious improvements in the possible range of experiments.

Each plug was first equilibrated in 300 ul of 1× digest buffer at room temperature for 20 minutes, before replacement of buffer with 100 ul of 1× digest buffer containing 200 units of restriction enzyme.

The next day cells were serum starved in RPMI without L-glutamine and phenol red for three hours followed by replacement of media with 90 μL PBS/HEPES buffer plus 10 μL of 25 μM Hydro-Cy3 for 15 min at 37°C, and subsequent imaging with a fluorescence microscope.

The appropriate negative controls for all immunostainings were established simultaneously by replacement of the polyclonal primary antibody by normal goat preimmune IgG diluted with phosphate buffered saline (PBS), containing 3%% bovine serum albumin at the same protein concentration as that used for the primary antibody.

It occurs in vertebrate skeletons by replacement of cartilage templates.

Reperfusion was simulated by replacement of culture medium (Fig.  2).

In this research, the sink condition was maintained by the addition of Tween 80 and frequent replacement of fresh buffer during the in vitro release experiment.

Heat-induced epitope retrieval was performed by heating the slides with sections in a microwave oven in a 0.02-M citrate buffer (pH 6.0) for a total of 10 minutes (two 5-minutes periods at 500 W, with replacement of evaporated buffer).

Complete replacement of phosphate buffer saline two times a week prevented pH changes of solutions that could result from increase of degradation products.