We confirmed the specificity of the effect by repeating the experiment using OPG siRNA as an alternative strategy to reduce OPG expression levels in MDA-MB-231 cells.
The results obtained by repeating the analyses using random effects models were highly consistent with the main findings (web table 5).
Second, we found that there may be some learning effects by repeating the same procedure.
Sensitivity analyses were performed by repeating the main computations using a fixed effect method.
Additional sensitivity analyses were conducted by repeating the main computations with the fixed effect method and by excluding trials with high risk of bias.
The effect was further confirmed by repeating the co-cultures to measure the BrdU-labeling index by flow cytometry, rather than by immunocytochemistry (Fig. 5).
Finally, because the CPRD does not include information on drug exposure in the inpatient setting, possibly leading to immeasurable time bias, 38 we assessed the effect of this bias by repeating the analyses after excluding patients who had been admitted to hospital during the current time window.
We performed a sensitivity analysis by repeating the main computations using a fixed-effect model and a subgroup analysis by population (locally advanced or localised).
We tested the impact of analyzing all delivery sites (50 maternity units in total) together by repeating the multivariate regression analyses with mixed-effects logistic regression models.
This paper reports an investigation of whether there is any adaptation to this effect, carried out by repeating the "after" phase of two previous studies of decreases in noise exposure.
