Sentence examples for by repeated steps of from inspiring English sources
Synthetic Atx1 peptides were purchased from Pepceuticals Limited (Nottingham-UK) and used after having been freed from residual trifluoroacetic acid by repeated steps of dissolution in sterile double-distilled water and lyophilization.
They eventually disappear when we keep removing monomers from the equilibrium, for example by repeated steps of pelleting and resuspending in fresh buffer.
Test the new mode' by repeating steps 1 to 3 of the "Booting to Single User Mode" section, then selecting "Rescue Mode", and pressing defer.add img);.
Prepare a shot of espresso by repeating Steps 2 through 4, above.
Make as many coils as you want by repeating step 3. Towards the end of your snake start narrowing the tail until you are at about half its width and at the middle of the above coil.
Next, DTC constructs a CDS tree, by repeating step 2 and step 3, from layer 1 to the last layer lmax of BFS tree.
Ionically bound CS-A was removed from the Sepharose by repeated washing steps of alternating 0.1 M acetate buffer, pH 4.5, containing 0.5 M NaCl and coupling buffer.
Repeated step-by-step cycles result in variously shaped shell structures without dies.
On the following day, one learning trial was given by repeating the steps of the habituation trial and 3 seconds after the door was closed, an unavoidable scrambled electric foot shock (0.5 mA for 2 s) was delivered through the grid floor of the dark compartment and the rat was removed 30 s later to its home cage.
The xylene was in turn removed by rotation in 1 ml of 100% ethanol for one hour followed by centrifugation and subsequent replacement of the supernatant with 90% ethanol for 45 min, and repeated by steps of 80% ethanol for 45 min and 70% ethanol for 45 min. Samples were freeze-dried, and total cellular DNA was extracted as described previously [ 29].
