Sentence examples for by removal of buffer from inspiring English sources

A wash step entails the resuspension of the concentrated beads with WB, followed by removal of buffer through the use of the MPC.

CTH1 was refolded by a stepwise removal of buffer containing urea ending up in 20 mM Citrate-phosphate buffer pH 4, 10% glycerol, 1 mM cysteine which yielded soluble protein.

The cells were washed three more times with cold HBSS followed by spin at 500  g and removal of buffer.

This resulted in better removal of buffer salts, as demonstrated by the lower scores on comp. 1 for the " plate, buffer, int.

Subsequently, cover slips were removed followed by removal of non-specific staining and background by a short immersion of the slides in Rinse buffer (DakoCytomation, Denmark) followed by 5 minutes incubation at 62°C in Wash buffer (DakoCytomation, Denmark).

These steps were found to be critical for the removal of buffer from the first dimension.

Experiments were terminated after 24 hours by removal of supernatants and addition of lysis buffer (Qiagen, Hilden, Germany).

For AFM analysis, Lys-C cleavage of procollagen was followed by removal of enzyme and propeptide fragments by buffer exchange into 10 mM HCl via multiple passes through a Millipore YM-100 microcon filtration unit.

Reactions were stopped by removal of the SNP saline, and addition of the kit assay buffer.

Briefly, the stepwise protein extraction was carried out by the removal of albumins and globulins with 500 µL of saline buffer (0·5 m NaCl, 10 m m Tris HCl, pH 7·5) followed by removal of cysteine-poor prolamins with 500 µL of 60%% (v/v) n-propanol solution and cysteine-rich prolamins with the same solution but containing 1%% (w/v) DTT.

This was accomplished by removal of the cell culture medium from the flasks followed by addition of 500 µl of lysis buffer plus 5 µl of β-mercaptoethanol from the GenElute Mammalian™ Total RNA Miniprep Kit (Sigma-Aldrich).