Sentence examples for by relative quantification using from inspiring English sources

The target gene expression levels were determined by relative quantification using the comparative CT method, also known as 2-∆∆CT method (comparative 2 [delta][delta]Ct method).

For both types of gene expression the target genes were quantified by relative quantification using the comparative CT method.

The relative levels of gene expression in fatty acid treated cells and the non-treated controls were calculated by relative quantification using gyrB as the endogenous reference gene.

Each mRNA expression was calculated by relative quantification using a normalization factor (geometric mean of two reference genes selected by geNORM program, http://medgen.ugent.be/genorm/) [35] and qBASE program for internal and external calibration and also for easy care of large RT-PCR datasets (http://medgen.ugent.be/qbase/).ugent.be/qbase/

Under- or overexpressed miRNAs can be analyzed by relative quantification using real-time PCR.

Gene expression levels were determined by relative quantification using the expression of histone H1.2 for normalization.

Analysis of SMARCB1/INI1 copy number by real-time quantitative PCR was performed by means of relative quantification using standard curve method [ 32], using TaqMan assays 4401631 (Applera) for RPPH1 endogenous control gene and Hs01497967_cn (Applera) for SMARCB1/INI1.

The presence of SMARCB1/INI1 hemyzigous deletion was confirmed also by Real-time quantitative PCR analysis of SMARCB1/INI1 copy number by means of relative quantification using standard curve method (not shown).

The relative amount of immunoprecipitated Abl protein was determined by immunoblotting and subsequent relative quantification using the Li-Cor Odyssey system.

Reaction efficiencies were used for relative quantification using the method as described by Pfaffl (Pfaffl, 2001).

The target cDNA was quantified by relative quantification method using a calibrator for primary tissue or respective controls for HT29 and SW707 cells.