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Infection was confirmed by relative quantification of DENV genomic RNA levels, which showed gradual increase in DENV genomic RNA over time (Fig. 4E). Figure 4: Expression pattern of AeCHD7 in DENV infected female Ae. aegypti.
Confirmation of the expression patterns of five clusters by relative quantification of transcript abundance using qPCR.
Hemolysis was measured by relative quantification of hemoglobin release in a spectrophotometer at 415 nm.
Parasite cDNA samples were standardized by relative quantification of an E. tenella β-actin PCR product.
In the present report, we have elucidated the SLP65 interactome and monitored the association kinetics by relative quantification of individual complex formations for various stimulation periods.
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In a first experiment, we tested the ability of the SCR-modules 19-20 (SCR1920) to interact with FH on the viral surface assessed by a viral lysis assay followed by a relative quantification of remaining viral RNA by real-time RT-PCR.
This may be due to the fact that the expression might be very low and thus be undetectable by immunodetection, as suggested by the relative quantification of the different transcripts.
Gene expression levels were determined by relative quantification using the expression of histone H1.2 for normalization.
Glycine levels determined by relative quantification showed a trend of higher levels in HER-2pos compared to HER-2neg tumors in the different ER and PgR status groups (p < 0.021 and q < 0.133).
Supplementary Fig. 2 Validation of gene expression differences by Taqman relative quantification of gene expression (GAPDH as internal control).
These changes in abundance are clearly observed by our relative quantification of the histone H4 immunoblot signal (Additional file 1: Figure S4).
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