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Se quantification in each sample was carried out by relative method using emission spectrum of NIST certified Se ICP standard solution SRM-13499).
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The standard deviation of the receiver DCBs estimated by the relative method was smaller than that of the single method.
The quantum yields of singlet oxygen generation were calculated by a relative method using methylene blue (in methanol) (Φ Δ = 0.50) as the reference.
In addition, we show by example that the relative method outperforms the absolute method if the calibration process is imperfect.
Heights determined in this way are useful in previously unmapped areas as a check on results obtained by faster relative methods, but they are not accurate enough for mapping developed areas or for engineering projects.
The densities of the mixture were measured using a 25-ml specific gravity bottle by relative measurement method with an accuracy of ±0.01 kg·m−3.
Data were analyzed by relative quantitation method using standard curve.
After normalization with GAPDH mRNA, the target gene mRNA level in a sample was quantified by relative quantification method.
The target cDNA was quantified by relative quantification method using a calibrator for primary tissue or respective controls for HT29 and SW707 cells.
The expression of these genes was calculated by relative quantification method with rhe L.chinense Actin housekeeping gene, which was also isolated through NGS sequences (data not shown), as a reference.
Using the comparative critical cycle (Ct) and by relative quantification methods (RQ), the expression levels of the target genes were normalized to the GAPDH endogenous control (ID-HS99999905_m1) and the relative abundance was calculated.
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