Sentence examples for by reference to the expression from inspiring English sources

cDNA samples isolated from non-manipulated H413 clone-1 cells were quantified by PicoGreen kit (Invitrogen) and then used for constructing standard curves (2 2000 pg) by reference to the expression of the house keeping gene encoding β-actin.

Gene expression for DAP3, ESRRA, ELK1 and RUNX1 was normalised by reference to the actin gene expression for each sample.

We quantified the variation in the range of expression by reference to the coefficient of variation (CV; a parameter shown in histogram/plot statistics) by FACS for EGFP-expressing cells.

Calculations to determine the relative level of gene expression were made by reference to the β-actin and SDHA in each sample, using the ΔCq method, and the results were depicted as arbitrary units.

Relative quantitation and normalization of the amplified targets were performed by the comparative ΔΔCt method in reference to the expression levels of the housekeeping gene ubiquitin [ 69].

Emblazoned with the words "Still the Enemy Within" – a reference to the expression used by Margaret Thatcher to describe the striking miners – the banner, in traditional crimson and gold, was stitched in the south London garage that doubles as Hall's workshop, and took him about 150 hours.

Low-scoring, taxonomically restricted orphans are most likely to be annotation artefacts: we tested this in the case of E. coli K12 by reference to online transcriptomic and proteomic expression data.

We quantified the PARP-1 expression by reference to purified recombinant PARP-1 protein (Biomol) standard curve (0 40 ng).

Expression was normalised by reference to Gapdh and in most cases the results were confirmed using Actb.

Values for angiotensinogen (AGT), prorenin and ACE mRNA expression were obtained by reference to standards curves generated using normal human liver, kidney and lung total RNA, respectively.

Values for AT1 and AT2 receptor mRNA expression were obtained by reference to a standard curve generated using normal human kidney total RNA.