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Data are expressed as pMol PAR per 10 cells by reference to cell counts or pMol per mg protein by reference to protein assay of the homogenate (Pierce assay; Thermo Scientific, Leicestershire, UK).
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Cell numbers were derived from OD values by reference to a standard cell number-OD curve.
Therefore, we feel that molecular pathology data can only be fully interpreted and understood by reference to normal mammary epithelial cell subtypes and the regulation of their differentiation.
Fold increases or decreases in each miRNA level of cell lines tested were determined by reference to the level of AZ-P7a cell line.
The rate of antigen production by the cells was estimated at 500 ng/day/10(6) cells by reference to a purified HBsAg standard.
Cellular identification was confirmed by reference to slides immunostained for cytokeratin (epithelial cells), actin (myoepithelial cells), and vimentin (fibroblasts) (results not shown).
Kinetic measurements of the formation of a coloured formazan derivative was used after nutrient broth addition to estimate initial cell concentrations by reference to Escherichia coli added as internal standard.
The Auto40 analyzer was initially intended for CD4 T cell enumeration based on primary CD4 gating and has been validated in Senegal for measurement of absolute CD4 T cell count by reference to the FACSCount system [ 11].
In patients with disseminated malignant melanoma an optimal method of immunization with irradiated tumour cells was developed by reference to an in vitro assay for circulating specific serum inhibitors of cell mediated cytotoxicity.
Absorbance was measured on a microplate reader at 450 nm and the amount of PrP in cell extracts was calculated by reference to a standard curve of recombinant murine PrP (Prionics, Zurich, Switzerland); its limit of detection was 0.05 ng/ml.
Absorbance was measured on a microplate reader at 450 nm and the amount of PrP in cell extracts was calculated by reference to a standard curve of recombinant murine PrP (Prionics, Zurich, Switzerland); its limit of detection was 50 pg/ml.
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