Sentence examples for by reductive methylation of from inspiring English sources

The apoA-I preparations were labeled with C to specific activities of ∼2 μCi/mg of protein by reductive methylation of lysine residues using [C]formaldehyde, as described previously; this level of trace labeling does not alter the lipid affinity of apolipoproteins.

Both drugs inhibit TS, the enzyme converting 2′-deoxyuridine to thymidylate by reductive methylation.

Cytb5-ops was purified from E. coli and C-labeled by reductive methylation.

The apoE variants were C-trace-labeled by reductive methylation as described previously.

The high estimate agrees well with the 225 reactive lysines detected by reductive methylation.

It restores MS activity by catalysing the reductive methylation of cob II)alamin on the inactivated MS in which S-adenosylmethionine is the source of the methyl group (Refs 118, 119).

Thymidylate synthase (TS) is a folate-dependent enzyme that catalyzes the reductive methylation of dUMP by 5,10-methylenetetrahydrofolate to form dTMP and dihydrofolate [ 1, 2].

The pyrimidine biosynthetic enzyme TS (thymidylate synthase; EC 2.1.1.45) catalyses the reductive methylation of dUMP by N N-methylenetetrahydrofolate, to form TMP and DHF (dihydrofolate) [ 1, 2].

Finally, regeneration of MeCbl is achieved via reductive methylation of cob II)alamin by SAM, again with inversion of stereochemistry for a net retentive process.

The protein was further purified by ion exchange chromatography and then submitted for reductive methylation of the lysine residues (Walter et al., 2006 ).

The SAM binding domain of MetH is involved in the reductive methylation of cob II)alamin, produced by adventitious oxidation of protein-bound cob(I alamin by molecular oxygen.