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In particular PCAP performance was obtained by reducing the stringency in overlap detection to tolerate more overlaps (using parameter -d 500), this parameter setting was necessary in order to generate reasonably long contigs.
Thus, by reducing the stringency of selection criteria for activity records, high average promiscuity rates were obtained.
This was further emphasised by reducing the stringency of the statistical analysis to p ≤ 0.08, which increased the number of genes detected by microarray specifically (not shown).
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Reducing the stringency by allowing there to be only two present calls instead of three produces higher numbers of present probes but the trends in the data are similar.
Reducing the stringency of the Align-GVGD scoring by restricting the alignments to the mammals-only subset (comprising sequences from nine species), four of the key domain rMS scored C0, and these were carried by four cases and one control.
Reducing the stringency of the model would increase the variation explained by the marker, but at the same time would result in more false positives.
Reducing the stringency of the fold change cut-off in our analysis to 2.5 fold induction revealed that a zeaxanthin epoxidase gene (ABA1), which catalyses the first step of ABA biosynthesis, was also induced by dicamba.
While it is possible that some mutation carriers were missed for technical reasons in the next generation sequencing or bioinformatics methods used, reducing the stringency of calling did not identify any additional variants, which were confirmed by Sanger sequencing.
As the SELEX rounds progressed, the stringency was increased by reducing the amount of selection target, reducing incubation times with the RNA pools, and increasing the number and length of washing steps.
We therefore increased the stringency of cross-linking by reducing the fixation temperature on ice.
Increasing the level of polymorphism of the chosen loci improves the stringency of paternity analysis, by reducing the rate of multiple assignments and type II errors.
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