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It has been suggested that immunoglobulins exert these actions either through their effects on cytokine production or directly by reducing cell adhesion molecule production [22, 23].
By reducing cell adhesion, it can seed new locations in the body.
Our results indicate that an important mechanism through which NRP1 antagonism may inhibit tumour cell migration is by reducing cell adhesion to ECM.
It has been suggested that immunoglobulins exert these actions either through their effects on cytokine production or directly by reducing cell adhesion molecule production [ 22, 23].
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Its role in reducing cell adhesion and increasing cell migration was demonstrated to be mediated by integrin ctVp3 [ 52].
Alterations in the expression of desmosomal components could contribute to the progression of the disease by modifying intracellular signal transduction pathways and/or by causing reduced cell adhesion.
By itself PAPC reduces cell adhesion physiologically to induce cell sorting, while FLRT3 disrupts adhesion excessively to cause cell dissociation.
In our studies, reduction of cellular packing density in MCC might be due either to cell killing by bortezomib or to reduced cell adhesion (or both); enhanced drug penetration is most likely related to reduced packing density although we cannot exclude some effect due to loss of cells from the surface of the MCC after drug treatment.
To enlighten the mechanism by which LFG knockdown reduces cell adhesion and enhances migration properties of NBL cells, we proceeded to LFG knockdown and performed a whole transcriptome analysis by Affymetrix expression arrays.
Interestingly, FZD7 signalling does not reduce cell adhesion by inducing C-cadherin internalisation, which is a common mechanism resulting in the endocytosis of cadherin complexes during cell migration.
In contrast, SMYGT reduced endothelial cell adhesion to the extracellular matrix (Additional file 1D), and image analysis revealed that 400 μg/mL of SMYGT reduced cell adhesion by 80.6%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com