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The reaction could be monitored by recording the decrease in the absorption at 377 nm.
Activity of APX (EC 1.11.1.11) was determined by recording the decrease in absorbance of ascorbate at 290 nm as described by Nakano and Asada (1986).
The activity of SOD was estimated by recording the decrease in absorbance of superoxide-nitroblue tetrazolium complex by the enzyme (Cavalcanti et al. 2004).
The reaction was initiated by adding the substrate D,L-glyceraldehyde and measured by recording the decrease in the absorbance of NADPH at 340 nm.
The swelling of mitochondria caused by an influx of solutes across the inner membrane was measured by recording the decrease in absorbance at 540 nm in a spectrophotometer (Bio-Rad, Hercules, CA, USA).
Because methylene blue and DCPIP accept two electrons in the oxidation step resulting in the color change of the dye, the reaction can be followed spectrophotometrically by recording the decrease of the absorbance.
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Cyprus (-1.2%), Portugal (-0.3%), Lithuania and Finland (both -0.2%) and Italy (-0.1%) recorded the only decreases.
The APX activity was recorded by following the decrease in absorbance at 290 nm for 3 min in 1 mL of a reaction mixture that contained 100 mM phosphate buffer (pH 7.5), 0.5 mM ascorbate, 0.2 mM H2O2 and 30 μL of enzyme extract.
Allele scoring and haplogroup inferences were based on the determination of the product-characteristic melting point temperature (Tm) values by on-line recording of the decrease in fluorescence-signal during a post-PCR temperature ramp from 60°C to 90°C (dissociation curve analysis, DCA, [29]).
The Kosice region recorded the biggest change, decreased by 13.74%.
The lower the coverage, the lower the completeness for all cases, since the proportion of cases expected to be captured by the disease recording system decreases.
More suggestions(15)
by recording the statement
by recording the diffraction
by recording the fault
by recording the extracting
by recording the indication
by recording the product
by recording the rate
by recording the emission
by recording the radiation
by recording the level
by recording the meeting
by monitoring the decrease
by recording the micronuclei
by recording the time
by recording the number
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