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It has been established by reconstituting a functional transposon from silent transposon fragments identified in the genomes of rainbow trout and Atlantic salmon (Ivics et al. 1997).
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A nonfunctional GUS NPTII transgene was corrected by transforming tobacco leaves with these geminivirus replicons, reconstituting a functional GUS copy.
In a single cycle of PRe-RDL, two halves of a parent plasmid, each containing a copy of an oligomer, are ligated together, thereby dimerizing the oligomer and reconstituting a functional plasmid.
Such a mechanism for reconstituting a functional UAA is known to occur in some mammalian mitochondrial transcripts [ 40].
The alternative scenario of a gene duplication followed by two alternative domains being lost individually in two duplicated genes which then reconstitute a functional channel is not at present well supported.
However, to reconstitute a functional RO system, all of the above components must work together in a coordinated manner.
To this end we reconstituted a functional E1-E2-Mdm2 ubiquitin ligasystemtem using purified proteins and added GST-FOXO4 as a substrate.
We performed BM transplantation experiments to assess the ability of LSK-CT and LSK-EVAi cells to reconstitute a functional thymus in Rag2-γc dKO sublethally irradiated mice.
However, close apposition of the fragments reconstitutes a functional GFP molecule and in this way, labels sites of synaptic contacts.
In preliminary experiments, we reconstituted the tetrameric PRC2 complex (Ezh2/EED/Suz12/RbAp48) in an insect cell expression system, following previous protocols established to reconstitute a functional PRC2 complex (Pasini et al., 2004; Ketel et al., 2005; Margueron et al., 2008).
This feature is beneficial when integrating transgenes, but the ability to excise a previously integrated transgene and reconstitute a functional landing site could be advantageous in other circumstances.
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