Sentence examples for by recognizing a phosphorylated from inspiring English sources

Slimb recruits the SCF complex to its substrates by recognizing a phosphorylated destruction motif.

Cells were fixed in 3.2% paraformaldehyde and stored in Methanol, followed by staining with antibodies recognizing Stat5 phosphorylated on Tyr697 (BD Biosciences), CrkL phosphorylated on Tyr207 (Cell Signaling), or Abl phosphorylated on Tyr412 (Cell Signaling) followed by a PE-conjugated goat anti-rabbit secondary antibody (Imgenex).

For immunohistochemical stainings an antibody recognizing eNOS, phosphorylated at Ser 1,177 (Biorbyt, Cambridge, UK) was applied.

We quantified the number of mitoses in the animal during pharynx regeneration by staining planarians with an antibody recognizing phosphorylated histone H3 at serine 10 (Hendzel et al., 1997; Newmark and Sánchez Alvarado, 2000).

Considering the potential role of AKT signaling in the disruption of epithelial architecture induce by Raf ER, we examined the activation state of AKT using an antibody that recognizes AKT phosphorylated at serine 473 by immunostaining.

However, there was no overexpression of Fyn, Yes, or c-Src in myeloerythroid cells from polycythemic recipients of JAK2 V617F-transduced Lyn −/− Hck −/− Fgr −/− BM, and little or no detectable activation of these Src kinases, as assessed by antibody recognizing the phosphorylated activation loop tyrosine in Src (Figure 6D).

In parallel with these results, we noted that the phosphorylation levels of Dnmt1, at residues preferentially recognized and phosphorylated by Akt and PKC (pDnmt1-PAS and pDnmt1-PPCS, respectively), increased when the tumor grade increased (Figure 3A).

It is not surprising that antibodies to phosphorylated αS Ser129 can cross-react with other phospho-proteins since this epitope can be phosphorylated by multiple kinases, such as casein kinases and polo-like kinases [ 24], and thus this phospho-epitope has a high level of homology to many phosphorylation sites in other proteins that are also recognized and phosphorylated by these kinases.

Then, sumo-NEMO is recognized and phosphorylated by ATM (Ataxia Telangiectasia Mutated), tagged by ubiquitination, which induces its release from ATM and its cytoplasmic translocation allowing NF- κB activation (Wu et al, 2006).

We generated an antibody that specifically recognizes Cry1 phosphorylated on S588 and measured a rapid increase in the presence of this phosphorylated species after exposure to DNA damage.

Importantly, the same motif is also recognized and phosphorylated by PknB (Table S1).