A sensible approach is to divide-and-conquer, where structural characterization of constituent subunits using standard techniques is followed by reassembly [7].
Artificially split MCM2 intein derived from Halorhabdus utahensis remained highly soluble and was capable of protein trans-splicing with excellent ligation kinetics by reassembly under high salinity conditions.
Chimeric A. thaliana glycosyl hydrolase (GH) gene libraries were assembled using a novel directed evolution strategy (TADSir: template assisted DNA shuffling and in vitro recombination) that promotes DNA recombination by reassembly of DNA fragments on unique gene templates.
Chromothripsis is an example of a genomic crisis, which is characterized by spontaneous catastrophic chromosome breakage followed by reassembly resulting in significant loss of chromosomal material [ 62].
Gaps identified in internalin genes were either closed by PCR and Sanger sequencing or by reassembly of the raw SOLiD™ system reads using improved versions of the de novo assembly tools.
Since this normally should be accompanied by significantly increased sequence coverage in the "collapsed" region, those effects are possibly amenable to automated pattern recognition and thus could become corrected by reassembly under modified conditions.
