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High throughput re-sequencing uncovers various genetic variations by mapping the sequencing reads of related individuals to the reference genome.
Validation of predicted SNPs by PCR and re-sequencing.
A total of 13,439 SNPs were discovered by BES re-sequencing.
However, our strategy for validation of putative SNPs by re-sequencing worked reasonably well.
More than 30,000 SNPs were detected in the coral sequences, and a subset of these validated by re-sequencing.
Sequencing errors in the revised genome assembly were identified by re-sequencing the genome of two different Nipponbare individuals using the Illumina Genome Analyzer II/IIx platform.
These SNPs were discovered by re-sequencing the DNA of eight individuals.
For example, more than 55 million SNPs were discovered in maize by genome sequencing and re-sequencing [ 17, 18] and 18.9 million SNPs were obtained by re-sequencing a core collection of rice accessions [ 19].
Annotation errors in two P. infestans genes due to sequencing related issues were corrected by re-examination of original sequence trace files at NCBI and by re-sequencing relevant regions.
1. 1536-SNP Illumina GoldenGate Assay: SNPs were selected from a SNP discovery pool generated by re-sequencing 20 diverse landraces of rice using Perlegen re-sequencing technology (www.oryzasnp.org; McNally et al. 2009).
Sequence gaps were closed by re-sequencing the end sequences of each contig, primer walking on linking clones, and by sequencing PCR products from genomic DNA.
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