Sentence examples for by real time amplification from inspiring English sources

A master mixture of QNASBA (Quantitative Nucleic Acid Sequence Based Amplification) was used to quantify marker proteins by real time amplification of the aptamers.

This detection can be performed by real time amplification using the specific Not I probe (Figure 1) or following gel analysis after restriction by NotI.

Each mutation was detected by real-time amplification followed by hybridization with two fluorescent probes designed with the sequence complementary to the wild-type sequences of gyr genes.

With the increase in sensitivity of the detection methodology, there is an increasing concern about the interpretation of positive NoV results by real-time amplification.

All primers pairs were designed using the Primer3 online software (http://frodo.wi.mit.edu/) and characterized by real-time amplification of a series of dilution of naked genomic DNA to determine linearity range and primer efficiency.

ChIP assays using an antibody against PPARγ followed by real-time amplification of an iNOS gene fragment flanking the recently identified PPRE [ 43] at iNOS gene showed a 119% increase in average mean amplification values in mice under HFHC diet with respect to those obtained in chow-fed mice (-100%) (p = 0.1).

Real time amplification was monitorized by addying SYBR Green I/fluorescein (Bio-Rad) to samples, using an iCycler iQ Multi-Color Real Time PCR Detection System equipped with the software iCycler iQ v 3.0, from Bio-Rad.

DNA samples were recovered and subjected to analysis by real time PCR amplification.

Methylation levels of three DNA fragments located within the CpG rich region (Additional file 1) were determined by Real Time PCR amplification of bisulfite treated DNA, followed by HRM profile analysis by CFX96™ Real-Time PCR Detection System, Bio -Rad Laboratories Inc. (Hercules, CA).

Methylation levels of DNA fragments located within the CpG island of the PHD1, PHD2, PHD3 and FIH genes (Additional file 2) were determined by Real Time PCR amplification of bisulfite treated DNA followed by HRM profile analysis by Light Cycler®480 Real-Time PCR System, Roche Diagnostics GmbH (Mannheim, Germany).

Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27.