A mutation frequency of one to five mutations per laccase gene was determined by sequencing ten randomly selected individual CotA clones.
There were two randomly sequenced sessions, one positive and one negative, which were determined by the valence of the slides displayed.
The quality of the library, composed by 2×106 single clones, was checked by sequencing fourteen randomly chosen clones, which showed high variability in nucleotide sequence (Figure 2A).
There were a total of 322 positions in the final dataset.> -wrap-foot>> -wrap-fooTajimaima's relative rate test was carried out by randomly comparing three phylogenetically distant sequences in each case by distributing them into three distinct group.
Accordingly, the heterozygosity of CBLs is greater than the number of segregating sites and present as multiple alleles.> -wrap-foot>> -wrap-fooTajimaima's relative rate test was carried out by randomly comparing three phylogenetically distant sequences of CBL proteins.
To avoid bias arising from unequal sampling size between locations, we homogenized the sample sizes for all locations from which more than eight sequences had been sampled, by randomly sub-sampling eight sequences from each of these.
We simulated the exome sequencing datasets by randomly extracting 75 million single-end 101nt sequencing reads from the 404,992 target regions on both the C57BL/6 J and SPRET/EiJ genomes.
Within each of these 35 datasets, diploid genotypes were manually constructed by randomly pairing two haploid DNA sequences (i.e., alleles), to generate a diploid genotype.
The representative sequences were selected by randomly choosing one from those which were similar to each other (approximately genetic distances <2.1%), isolated from the similar host (avian or a species of mammals) in the same year and in the same country.
To illustrate this feature, we have simulated unassembled Illumina data using MetaSim [ 21] by randomly sampling one million 100 base pair sequences from the complete genome sequences shown in Figure 3A and applying an Illumina error model.
