Sentence examples for by random shearing using from inspiring English sources

After purification, mRNA was fragmented by random shearing using heat treatment in the presence of Mg2+ ions.

Because DNA fragmentation for SIMPLE occurs by random shearing of genomic DNA by sonication, SIMPLE is able to detect L1 elements that may be located too distally from restriction enzyme cut sites used by other high-throughput sequencing methods [ 25, 26].

Each RAD tag has one end defined by the restriction enzyme recognition site, and the other end defined by random shearing.

Briefly, cells were fixed in 1 % formaldehyde and lysed followed by sonication shearing using the Bioruptor sonicator (Diagenode, Belgium).

Each Tn integration site was generally represented by multiple reads, and these reads usually varied in the length of the Salmonella sequence due to the random DNA shearing used in the sequencing protocol.

The gDNA inserts of PGB02 and PGB04 were isolated by gel purification and sheared using a nebulizer (Invitrogen).

Colony 356a gDNA was sheared using HydroShear (speed setting 16; 20 cycles) into random fragments of 6 10 kb.

The cells were sheared using a syringe, and lysates cleared by centrifugation at 10 000  g for 30 min at 4°C.

DNA is sheared using a small gauge needle, and insoluble material is precipitated by centrifugation.

DNA protein complexes were sheared using an enzymatic shearing kit (Active Motif) to yield DNA fragments below 800 bp, as determined by agarose gel electrophoresis.

While some signal may be gained by reducing the stability of secondary structure in the target molecule, random shearing by its nature creates a mixture of targets that may have substantially different affinities.