Sentence examples for by random priming using from inspiring English sources

Briefly, 400 ng of test and control DNA were labelled by random priming using the BioPrime Array CGH Genomic Labelling System (Invitrogen, Life technologies, Carlsbad, CA, US).

The DNA fragment was labeled with 32P by random priming using a Megaprime DNA labeling kit (GE Healthcare).

32P-labeled PHO5 upstream and open reading frame probes were prepared by random priming using a 500 bp NotI/BamHI fragment and a 687 bp ScaI/SalI fragment as template, respectively.

Linear-amplified DNA was labeled with amino-allyl-dUTP by random priming using the BioPrime DNA Labeling kit, (Invitrogen Co .. Amino-allyl-dUTP-containing DNA was coupled to the Cyanine dyes Cy5 or Cy3 (Amersham).

The human p16INK4a gene-coding region was used as probe labeled with [α-32P] dCTP (Yahui, Beijing, China) by random priming using the Prime-a-Gene Labeling System (Promega).

These were probed with a 1.8 kb Bam HI HIS3 genomic clone corresponding to sequences from 469 bp upstream of the HIS3 open reading frame to 634 bp downstream that had been labeled with 32P by random priming using a Megaprime DNA labeling kit (GE Healthcare).

The probes were labeled by random priming (23 ) using a DNA labeling kit (Bethesda Research Laboratories) and α-P‑deoxycytidine triphosphate (3,000 Ci/mmol, Amersham Biosciences).

The probes were labeled with [α-32P]-deoxycytidine triphosphate by random prime synthesis using Amersham Rediprime II Random Primer Labelling System (GE Biosciences).

Radioactive labelling was performed by random hexamer priming using Ready-to-go DNA labelling beads (Amersham Pharmacia Biotech, USA) and α32P-dCTP.

Complementary cDNA was synthesised from 2 μg of RNA by random hexamer priming using MMLV reverse transcriptase (Promega).

The cDNA was synthesised with 2.0  μg of mRNA by random hexamer priming using the AdvantageTM RT-for-PCR kit (Clontech, Palo Alto, CA, USA).