Sentence examples for by random priming of from inspiring English sources

The implication for first strand priming using oligo-dT followed by random priming of the second strand is that amplified RNA will likely be biased toward the 3' end of the message since initial priming of the RNA is anchored with oligo-dT and thus potentially under-represents coding sequences found at the 5' of any given operon.

The 5S rRNA gene probe, labeled with a32P-dCTP, was generated by random priming of a full-length 5S gene repeat amplified by PCR from clone pCT4.2 [38].

First-strand cDNA synthesis was carried out by random priming of the total RNA using a random primer mixture (Invitrogen) and reverse transcriptase with superscript 3 (Invitrogen).

Where SELEX was primarily used as a tool to analyze the structure of RNA aptamers to a given ligand, Genomic SELEX transforms the method into a discovery tool to screen endogenous RNAs by starting from a pool derived by random priming of the genome of interest.

Non-cloned libraries were prepared by random priming of polyA + mRNA and size fractioning.

cDNA libraries were constructed by random priming of chemically sheared poly A captured RNA.

This finding might be explained by the different techniques of reverse transcription that is carried out by random priming for the qPCR and by oligo-dT for the microarray experiments.

The mRNA then underwent a mild heat fragmentation followed by random priming for first-strand synthesis.

The purified mRNA was then subjected to a mild heat fragmentation followed by random priming for first-strand synthesis.

Aliquots of 250 μl of DNA were labeled by random priming with 2 μl of P-dCTP.

The probe was prepared from BamHI-digested full length TYLCCNV and labeled by random priming in the presence of [α-32P]dCTP.