Sentence examples for by random primer using from inspiring English sources

32P-labelled probe was synthesized by random primer using the DECAprime™ II kit from Ambiom (Austin, TX, U.S.A) following the manufacturer's instructions.

First-strand cDNA was synthesized by random primer using the SuperScript III RT kit (Invitrogen, Carlsbad, CA) following the manufacturer's instruction.

For microarray analysis, the quality of RNA from IP material was initially analyzed on a Agilent Bioanalyzer 2110 (Agilent Technologies, Böblingen, Germany) before mRNAs were reverse transcribed by random priming using oligo dT primers.

Adli et al. reported a modified protocol to realize the ChIP-Seq using 10 000 cells by revising the random primers used in amplification to reduce the primer self-annealing, with an optimized PCR condition to cover the GC-rich regions.

Probe DNA was labeled with [P]dCTP by random primer extension using DecaLabel™ DNA Labeling Kit (MBI Fermentas, Vilnius, Lithuania).

Probes were generated from full length mtDNA (16.5 kb) by random primer labeling using the DIG System (Roche Diagnostics).

Among 40 random primers used in this experiment, 31 primers generated the same DNA bands type, and nine primers generated a different DNA band type.

IS CR and sul2 PCR product amplified with primers CRF/CRFF-r were labeled with P-ATP by random primer extension by using a commercially available kit (Stratagene, Amsterdam, the Netherlands) according to the manufacturer's instructions.

The first strand cDNA was synthesized by random hexamer-primer using the mRNA fragments as templates.

Equal amounts of total RNA (1 μg) were reverse transcribed by random hexamer primers using the Maxima Enzyme Mix (Fermentas).

For RT-PCR, RNAs were reverse transcribed by random hexamer primers using Superscript III (Invitrogen) according to the manufacturer's instructions.