Sentence examples for by random mutagenesis using from inspiring English sources

Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5 40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L).

Often phenotypes are intentionally created by random mutagenesis using chemicals, radiation or insertional mutagenesis.

Using S. cerevisiae Ethanol Red as platform, the by now classical approach commenced with rational metabolic engineering through transformation with a cassette containing the XI gene from Clostridium phytofermentans, followed by random mutagenesis using EMS with hydrolysate and xylose as selective criteria.

Insouciant, oblivious and heedless were generated by germ-line, random mutagenesis using ENU (N-ethyl N-nitrosourea) where their phenotypes result from loss of functional TLR6, CD36 and CD14, respectively [22], [16], [21].

In the present study, we found that the V209 residue was related to Rd-hydrolyzing activity by analysis of variant enzymes constructed from random mutagenesis using error-prone PCR.

Random Mutagenesis Using a Mutator Strain / Ranjan Sen -- 30.

To understand the thermal adaptation strategy of proteins, comparative studies among members of a protein family using site-directed mutagenesis as well as laboratory evolution via random mutagenesis using error-prone PCR will provide valuable information.

The c-Photina gene (mutant 12, Patent Application EP06000171) was obtained by random mutagenesis from the gene Clytin (GenBank accession number Q08121) using the GeneMorph II Random Mutagenesis kit (Stratagene, La Jolla, CA, USA) following the supplier's instructions with the following primers: Upper: GATGACGACGACAAG-ATGGCCGACACCGCCAG Lower: GAGGAGAAGCCCGGT-TTATCAAGGACACGAAGT.

Cellulases are produced industrially using T. reesei strains genetically modified by random mutagenesis or by targeted genetic modifications such as introducing strong inducible promoters, increasing the gene copy numbers or removing undesired secreted proteins [ 15].

While strain improvement was traditionally accomplished by random mutagenesis, a detailed understanding of cellulase regulation can only be gained using recombinant technologies.

More recently, this approach has been used to interrogate a collection of conditional mutants generated by random mutagenesis to identify canonical splicing mutants.