Sentence examples for by random integration using from inspiring English sources

We show that targeted delivery of an anti-CD19 CAR cassette to the CCR5 locus using a recombinant AAV homology template and an engineered megaTAL nuclease results in T cells that are functionally equivalent, in both in vitro and in vivo tumor models, to CAR T cells generated by random integration using lentiviral delivery.

Furthermore, when R4 attB and attP sites are placed into the human genome, either by random integration or at a specific sequence by using the φC31 integrase, they act as targets for integration of incoming plasmids bearing R4 att sites.

Genomic targets enabling FLP RMCE can either be established by random integration (electroporation and transfection; Turan et al. 2013) or using the scout functions and integration preferences of retroviral vectors (Kuehle et al. 2014).

Transgenesis by random integration of naked DNA has proven much more efficient for gene supplementation, whether using PNI or SCNT.

HGn39D was generated by random integration of an enhancer-trap transgene [28].

Secondly, Cre transgenic mouse strains are mostly generated by random integration of a DNA construct comprising promoter and Cre cDNA into the host DNA.

Transgenic fly lines were obtained by random integration of the UAS-Myc-ets21c LONG transposon (Genetic Services).

Protein trap screens utilise artificial reporter-encoding exons to generate fluorescent fusion proteins by random integration into the genome.

The Tg[gSAGFF202A] line was generated by random integration of a gene-trap construct (Asakawa et al., 2008).

The ET krt4 EGFP sqgw57A line was generated by random integration of an gene-trap construct (Kondrychyn et al., 2011).

To remove any possible confounding positional effects generated by random integration of Tau transgenes, we adopted φC31-mediated site-specific integration to standardise the genomic integration locus (Groth et al., 2004).