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In fact, transgene expression levels in these three lines were comparable to those in the remaining transgenic plants, suggesting that the observed delay in growth is not related to heterologous expression of the yeast transporters, but rather to the regions of the Arabidopsis genome disrupted by random insertion of the transgenes.
This inconsistency might be caused by random insertion of the transgenic construct into the chromosome, a common unintentional outcome of the transgene approach.
The P. aeruginosa STM library [19] was created by random insertion of the transposon pUTmini-Tn5 into the genome of PA01 strain from Holloway et al [46].
Protein trapping offers an alternative, powerful approach to abolish gene function by random insertion of DNA.
A mutant library was constructed by random insertion of mini-Tn 10 Km in the E. coli chromosome.
The Mpv17 gene was first identified in laboratory mice created by random insertion of a retroviral construct in the genome of mouse embryonic stem cells (20).
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The mutagenic potential caused by random insertions of any transposon remains the greatest concern for their advancement to clinical applications.
In earlier techniques, the library of E. coli strains with different positions for site-specific (Flp-dependent) integration was constructed by random insertions of corresponding recombinogenic (FRT) sites by the Tn5-system [ 3].
NHEJ might alter the DSB site by random insertion or deletion of nucleotides of varying length.
The interior of the castle has been damaged by the random insertion of trenches by treasure hunters since the early 18th century.
In the different transgenic mouse lines, such as RIP-Cre, functional differences may result from insertional mutagenesis caused by the random insertion of the transgene, usually as large concatemers.
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by random relocation of
by random amplification of
by random shuffling of
by random ligation of
by random sampling of
by random shuffle of
by random motion of
by random nucleation of
by random assignment of
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by random sequencing of
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by random permutation of
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