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Relative gene-expression analyses were performed by quantitative reverse transcriptase PCR (qRT-PCR).
In this study, a two-step ultrafiltration (UF) procedure was used to concentration of VHSV in seawater, providing samples that were tested for infectivity by cell culture and the presence of VHSV by quantitative reverse transcriptase PCR (qRT-PCR) methods.
Microarray results for significantly changed genes were confirmed by quantitative reverse transcriptase PCR (qRT-PCR) (Figure 1B).
In the present study we investigated overexpression of ETV1 by quantitative reverse transcriptase reaction (QPCR) in a novel cohort of 66 prostate cancers.
The relative level of expression of the TbMyo1 mRNA was estimated by quantitative reverse transcriptase PCR (QRT-PCR) using the Brilliant® SYBR Green qRT-PCR Master Mix Kit (1-step) from Stratagene in an MxPro 3000 instrument (Stratagene).
Partially, this induction occurred at the transcriptional level, because GS mRNA determined by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) increased 2.38-fold±0.38 (N = 3; p<0.05) after induction by 1 μM dexamethasone within 24 h (Table 1).
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Transcript levels of genes encoding chondrogenic factors were determined by quantitative reverse transcriptase-polymerase chain reaction.
In addition to the profiling analysis, the expression levels of ten DEGs were validated by quantitative reverse transcriptase-PCR.
Of these, only nine genes were confirmed by Quantitative Reverse Transcriptase-Polymerase Chain Reaction (QRT-PCR), all of which were downregulated in narcolepsy.
We analyzed ECT2 expression in OSCC-derived cell lines and primary OSCCs compared with matched normal tissue (n = 96) by quantitative reverse transcriptase-polymerase chain reaction, Western blot, and immunohistochemistry.
In this study, the miRNA profile associated with the development of MPM was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of biopsies freshly collected from patients with MPM and from healthy subjects used as controls.
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